Alfisol, Item 003: LMMpro, version 2.0
The Langmuir Optimization Program
The Michaelis-Menten Optimization Program

The Michaelis-Menten

The Michaelis-Menten Equation & Enzyme Kinetics --- an overview.

The Michaelis-Menten Equation was introduced in 1913. It correlates the initial enzyme reaction rate (v) with the amount of substrate present (S). This enzyme kinetics equation can be derived as follows:

If KM is known, if Vmax is known, and if the reaction is as described above, then the reaction rate (v) can be predicted for any initial substrate concentration (S). The two parameters (Vmax and KM) are obtained by regression analysis of known S and v values. It is worth noting that when S = KM, the observed reaction rate (v) will equal Vmax/2.

<Reference = CTT-3>